Diffinity Genomics has developed an innovative new technology that removes
undesirable impurities from PCR reactions leaving you with nothing but purified
DNA in solution — fast!
DiffinityTM technology effectively removes dNTPs, primers, and primer dimers while providing greater than 80% recovery of pure DNA
fragments ranging in length from 150bp to 10Kb. It dramatically reduces the amount of time to purify and prepare PCR reactions for use
in downstream applications, such as DNA sequencing, SNP analysis, and microarray printing.
The functional pipette tip will fit on most conventional single or multiple, manual or automated laboratory pipettors. Samples containing
DNA and PCR impurities are aspirated into the tip where the impurities are adsorbed onto proprietary surfaces within the tip by mixing
for approximately one minute. Expelling the solution yields purified DNA, the desired product.
Product Comparion Chart
|
|
Diffinity
RapidTip®
RT025-048
RT025-048L
RT025-096
RT025-096L
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Diffinity
RapidTip®2
RR050-048
RR050-048L
RR050-096
RR050-096L
|
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Impurities
removed
|
dNTP
|
Yes
|
Yes
|
|
Primer
|
Yes
|
Yes
|
|
Primer-dimer
|
Yes
|
Yes
|
|
DNA polymerase
|
No
|
Yes
|
|
Other Enzymes
|
No
|
No
|
|
Tip Format
|
Tip Formats Available
|
Universal
Rainin LTS®
Robotic (Contact us)
|
Universal
Rainin LTS®
|
|
Sample Volume
|
PCR Sample Volume
|
25 microliters
|
50 microliters
|
|
Fragment Size
|
Fragment Size Cutoff
|
Removes < 40bp Retains > 150bp to 10Kb
|
Removes <
40bp Retains > 150bp to 10Kb
|
|
Downstream
Applications
|
Optimized for
these downstream applications
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PCR purification
prior to Sanger sequencing
|
PCR purification
prior to restriction digestion, TA cloning
PCR purification prior to Sanger sequencing
|
