Inspire™ HILIC Columns 은 HPLC 컬럼입니다.
Features of Inspire™ HILIC Columns
• Unique selectivity and increased retention for highly polar compounds
• Increased sensitivity and lower detection limits with microbore
• Enhanced retention for hydrophilic / polar compounds
• Increased laboratory throughput and productivity
• Superior batch-to-batch reproducibility
• Suitable for the separation of hydrophilic compounds such as polar acids and bases, polar compounds, nucleosides, oligonucleotides, amino acids, peptides,
and water-soluble vitamins
HILIC is normal phase chromatography of polar and ionic compounds under RP conditions. It combines the characteristics of three methods in LC-RP, NP, and
IC. InspireTM HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of polar compounds onto the stationary
phase for increased retention. Separation is achieved through the partitioning of polar solutes from the high concentration, water-miscible, organic mobile
phase into the hydrophilic surface environment. Polar solutes exhibit increased retention, and elute in the order of increasing hydrophilicity. HILIC is ideal for
separating and retaining polar compounds that may not retain on traditional RP packings. Unlike reversed-phase columns, InspireTM HILIC columns retain highly
polar compounds with only small amounts of water in the mobile phase. These more volatile mobile phases increase sensitivity with microbore applications.